Incorporation of acetate and sucrose by Torulopsis utilis.
نویسندگان
چکیده
The oxidative assimilation of carbon compounds by nonproliferating yeast cells has been studied by several workers. Winzler (1940) found that when Saccharomyces cerevisiae was allowed to oxidize sodium acetate only 66 per cent of the substrate was oxidized; the remainder was incorporated into the cell. Winzler and Baumberger (1938) found that when glucose was the substrate only 26.5 per cent of the substrate was oxidized; the remaining 73.5 per cent was assimilated. Since these workers found a respiratory quotient of one, they postulated that the cellular material being formed was carbohydrate. Other workers (Smedley-Maclean and Hoffert, 1923; Lindegren, 1945) have reported that considerable quantities of lipides are formed. The turnover of this stored material has been studied by Yemm and Folkes (1954). They found that when Torulopsis utilis was incubated with sucrose the intracellular carbohydrate increased. When this yeast was subsequently incubated with (NH4)2HP04, the cellular carbohydrate decreased and ammonia was taken up by the cells. Similar results were found with baker's yeast (Yemm and Folkes, 1954) and Escherichia coli (Holme and Palmstierna, 1956). This paper reports experiments conducted with T. utilis incubated in nitrogen-free media containing either sucrose and acetate or acetate alone. An attempt has been made to determine what compounds are formed and how much of the intracellular carbon is metabolized to carbon dioxide.
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Protein enrichment of an Opuntia ficus-indica cladode hydrolysate by cultivation of Candida utilis and Kluyveromyces marxianus
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ورودعنوان ژورنال:
- Journal of bacteriology
دوره 81 شماره
صفحات -
تاریخ انتشار 1961